Autoradiography At the Cellular Level. Physical Techniques by Brigitte Schultze

By Brigitte Schultze

Show description

Read Online or Download Autoradiography At the Cellular Level. Physical Techniques in Biological Research PDF

Best physical books

Human Demography and Disease

Human Demography and affliction deals an interdisciplinary and built-in standpoint at the dating among historic populations and the dynamics of epidemiological strategies. It brings the innovations of time-series research and desktop matrix modeling to historic demography and geography to extract exact info about the oscillations in births, deaths, migrations and epidemics.

Seasonality and Human Ecology (Society for the Study of Human Biology Symposium Series)

Seasonality has results on quite a lot of human features and actions, and is necessary within the knowing of human-environment relationships. during this quantity, exclusive participants together with human biologists, anthropologists, physiologists and nutritionists contemplate a number of the other ways during which seasonality affects human biology and behaviour.

Mathematical and Physical Modelling of Microwave Scattering and Polarimetric Remote Sensing: Monitoring the Earth’s Environment Using Polarimetric Radar: Formulation and Potential Applications

Radar know-how is more and more getting used to observe the surroundings. This monograph presents a evaluation of polarimetric radar options for distant sensing. the 1st 4 chapters disguise the fundamentals of mathematical, statistical modelling in addition to actual modelling in keeping with radiowave scattering concept.

Mechanisms of Psychological Influence on Physical Health: With Special Attention to the Elderly

In recent times principles concerning the effect of mental prestige on actual health and wellbeing were increased from instinct and scientific lore to a valid zone of clinical learn. Mechanisms of impact are, certainly, assorted. a few of this effect is oblique, accounted for just by the instrumental support that social engagement, linked to reliable psychological overall healthiness, provides humans; for instance, psychologically "healthy" everyone is most likely likely to conform to drugs regimens and interact in different healthy behaviors to a better volume than those who find themselves socially withdrawn or psychologically volatile.

Additional resources for Autoradiography At the Cellular Level. Physical Techniques in Biological Research

Sample text

Development and Fixation Manufacturer's recommendations should be followed for development. For most of the thin emulsions usable for autoradiography and stripping film 4-6 min development in Kodak D-19 is suitable. Leblond et al. (1963) and Kopriwa and Leblond (1962) have determined the influence of differ­ ent developers and of developing time on grain counts in autoradiograms 3 of H-labeled sections. They found that there is no marked difference in grain counts with the various developers. Since the background increases slowly during the first period of development, but later on rises quickly with increasing time, the development time should be kept short.

Schultze and Hughes 125 (1965) showed that I-labeled ribonuclease injected into mice intra­ venously is localized in the epithelia of the proximal tubules in the kidney. 125 Kay es et al. (1962) used I in electron microscopic autoradiography of radioiodine in the thyroid, obtaining good pictures of ultrastructural details without removing the remaining gelatin. Similar studies are per­ formed by Kayes et al. (1962), Stein and Gross (1963), Dohlman et al. (1964), Sheldon et al. (1964), Lupulescu et al.

TECHNIQUES USING CRYOSTAT SECTIONS AND Low EXPOSURE TEMPERATURES In recent investigations cryostat sections have been used for tracing water-soluble substances autoradiographically. Oster et al (1955) first introduced a method of cutting unfixed frozen tissue with the freezing microtome, applying the emulsion without thaw­ ing, and exposing the autoradiograms at — 20°C. Further attempts have been made to prevent loss or diffusion of water-soluble substances by 44 2. , 1958; Taugner and Wagenmann, 1958; Huang, 1960; Reinholz et al, 1960; Novek, 1962).

Download PDF sample

Rated 4.85 of 5 – based on 28 votes