By Brigitte Schultze
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Development and Fixation Manufacturer's recommendations should be followed for development. For most of the thin emulsions usable for autoradiography and stripping film 4-6 min development in Kodak D-19 is suitable. Leblond et al. (1963) and Kopriwa and Leblond (1962) have determined the influence of differ ent developers and of developing time on grain counts in autoradiograms 3 of H-labeled sections. They found that there is no marked difference in grain counts with the various developers. Since the background increases slowly during the first period of development, but later on rises quickly with increasing time, the development time should be kept short.
Schultze and Hughes 125 (1965) showed that I-labeled ribonuclease injected into mice intra venously is localized in the epithelia of the proximal tubules in the kidney. 125 Kay es et al. (1962) used I in electron microscopic autoradiography of radioiodine in the thyroid, obtaining good pictures of ultrastructural details without removing the remaining gelatin. Similar studies are per formed by Kayes et al. (1962), Stein and Gross (1963), Dohlman et al. (1964), Sheldon et al. (1964), Lupulescu et al.
TECHNIQUES USING CRYOSTAT SECTIONS AND Low EXPOSURE TEMPERATURES In recent investigations cryostat sections have been used for tracing water-soluble substances autoradiographically. Oster et al (1955) first introduced a method of cutting unfixed frozen tissue with the freezing microtome, applying the emulsion without thaw ing, and exposing the autoradiograms at — 20°C. Further attempts have been made to prevent loss or diffusion of water-soluble substances by 44 2. , 1958; Taugner and Wagenmann, 1958; Huang, 1960; Reinholz et al, 1960; Novek, 1962).